Insulin-Like Growth Factor 1 (IGF-1) Gene Polymorphism in Some Goat Breeds Reared in Turkey

Mahamat Ali Amine Ouchar; Birol Dağ; İbrahim Aytekin

doi:10.5281/zenodo.8238374

Yazarlar

Mahamat Ali Amine Ouchar Institut de Recherches en Élevage pour le Développement (IRED), Tchad https://orcid.org/0000-0003-1159-9971
Birol Dağ Selcuk University, Faculty of Agriculture, Department of Animal Science, Postal Code: 42130, Department of Zootechnie, Konya, Türkiye https://orcid.org/0000-0002-6123-2724
İbrahim Aytekin Selcuk University, Faculty of Agriculture, Department of Animal Science, Postal Code: 42130, Department of Zootechnie, Konya, Türkiye https://orcid.org/0000-0001-7769-0685

DOI:

https://doi.org/10.5281/zenodo.8238374

Anahtar Kelimeler:

IGF-1, HaeIII polimorfizm, PCR, RFLP, keçi

Özet

Bu araştırmada amaç dokuların büyümesi ve kas gelişmenin düzenlenmesinde aday gen olarak kabul edilen İnsülin Benzeri Büyüme Faktörleri -1 (IGF-1) geninin ekzon 4 ve İntron 4 bölgesindeki HaeIII polimorfizmini Türkiye’de yetiştirilen 6 farklı keçi ırkında belirlemektir. Araştırmada 52 baş Kıl, 51 baş Tiftik, 50 baş Honamlı, 50 baş Halep, 50 baş Saanen ve 50 baş Kilis ırkı olmak üzere toplam 303 baş keçide GH1 geni HaeIII polimorfizmi (363 bç) Restriksiyon Fragman Uzunluk Polimorfizmi (RFLP) yöntemi ile araştırılmıştır.

IGF-1 geni HaeIII enzim kesimi sonucu iki allel (A ve B) ve üç genotip (AA, BB ve AB) belirlenmiştir. Kıl, Tiftik, Honamlı, Halep, Saanen ve Kilis keçi ırkları için A ve B allel frekansları sırasıyla 0.3558 ve 0.6442, 0.5784 ve 0.4216, 0.2100 ve 0.7900, 0.7300 ve 0.2700, 0.7800 ve 0.2200, 0.2900 ve 0.7100 olarak bulunmuştur. AA, AB ve BB genotip frekansları bakımından ise sırasıyla 0.250, 0.212 ve 0.538 (P<0.05), 0.451, 0.255 ve 0.294 (P<0.05), 0.060, 0.300 ve 0.640 (P>0.05), 0.600, 0.260 ve 0.140 (P<0.05), 0.700, 0.160 ve 0.140 (P<0.05), 0.200, 0.180 ve 0.620 (P<0.05) olarak bulunmuştur. Bütün ırklar dikkate alındığında A ve B allel frekanslarını 0.4901 ve 0.5099, AA, AB ve BB genotip frekanslarını ise 0.376, 0.228 ve 0.396 (P<0.05) olarak tespit edilmiştir. Honamlı keçisi dışındaki ırkların Hardy-Weinberg dengesinde olmadığı belirlenmiştir (P<0.05).

Referanslar

Anonim, 2018. http://www.tuik.gov.tr/Start.do Access date: 03.05.2018.

Anonymous, ‎‎(2017‎), Capra hircus breed San Clemente chromosome 5, ASM170441v1, whole genome shotgun sequence. https://www.ncbi.nlm.nih.gov/nuccore/NC_030812.1?report=genbank&from=64862983‎ &to=64943172 &strand=true. Erişim Tarihi: [05.07.2017] ‎.

Alakilli, S. Y., Mahrous, K. F., Salem, L. M. ve Ahmed, E. S., 2012, Genetic polymorphism of five genes associated with growth traits in goat, African Journal of Biotechnology, 11 (82), 14738-14748.

Cohen, L. E., Wondisford, F. E. ve Radovick, S., 1996, Role of Pit-1 in the gene expression of growth hormone, prolactin, and thyrotropin, Endocrinology and metabolism clinics of North America, 25 (3), 523-540.

FAO (2017). Food and agriculture data. http://www.fao.org/statistics/en/. Access Date: [19.06.2019].

Kaymakcı, M., Koşum, N., Taşkın, T., Akbaş, Y‎, ‎(2006)‎, A research on determination of some yield characteristics in Menemen sheep‎, Ege University Faculty of Agriculture Journal, 43(1): 63-74.‎

Kaymakcı, M. A., Y‎, ‎‎(1997)‎, Goat breeding, Ege University Faculty of Agriculture,Bornova, İzmir, 1997, 294.

Kurdistani, Z. K., Rostamzadeh, J., Rashidi, A., & Davis, M. E. (2013). Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats. Small Ruminant Research, 111(1-3), 10-15.

Lan, X. Y., Pan, C. Y., Chen, H., Lei, C. Z., Liu, S. Q., Zhang, Y. B., ... & Hu, S. R. (2007). The HaeIII and XspI PCR-RFLPs detecting polymorphisms at the goat IGFBP-3 locus. Small ruminant research, 73(1-3), 283-286.

Liu, W., Fang, G., Fang, Y., Tian, K., Huang, X., Yao, X., ... & Xin, Y. (2010). The polymorphism of a mutation of IGF-1 gene on two goat breeds in China. Journal of Animal and Veterinary Advances, 9(4), 790-794.

Li, M., Li, Y., Lu, L., Wang, X., Gong, Q., & Duan, C. (2009). Structural, gene expression, and functional analysis of the fugu (Takifugu rubripes) insulin-like growth factor binding protein-4 gene. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 296(3), R558-R566.

Li ve Crenshaw, E., 3d, Rawson EJ, Simmons DM, Swanson LW & Rosenfeld MG‎, ‎1990 ‎, Dwarf locus mutants lacking three pituitary cell types result from mutations in the ‎POU-domain gene pit-1‎, Nature, 347, 528-533.‎

Shareef, M., Basheer, A., Zahoor, I., & Anjum, A. A. (2018). POLYMORPHISMS IN GROWTH HORMONE (GH) AND INSULIN-LIKE GROWTH FACTOR-1 (IGF-1) GENE AND THEIR ASSOCIATION WITH GROWTH TRAITS IN BEETAL GOAT. Pak. J. Agri. Sci, 55(3), 713-720.

Mikawa, S., Yoshikawa, G. I., Aoki, H., Yamano, Y., Sakai, H., & Komano, T. (1995). Dynamic aspects in the expression of the goat insulin-like growth factor-I (IGF-I) gene: diversity in transcription and post-transcription. Bioscience, biotechnology, and biochemistry, 59(1), 87-92.

Miller, B. H., & Gore, A. C. (2001). Alterations in hypothalamic insulin‐like growth factor‐I and its associations with gonadotropin releasing hormone neurones during reproductive development and ageing. Journal of neuroendocrinology, 13(8), 728-736.

Reinecke, M., & Collet, C. (1998). The phylogeny of the insulin-like growth factors. In International review of cytology (Vol. 183, pp. 1-94). Academic Press.

Schibler, L., Vaiman, D., Oustry, A., Giraud-Delville, C., & Cribiu, E. P. (1998). Comparative gene mapping: a fine-scale survey of chromosome rearrangements between ruminants and humans. Genome Research, 8(9), 901-915.

Alakilli, S. Y., Mahrous, K. F., Salem, L. M., & Ahmed, E. S. (2012). Genetic polymorphism of five genes associated with growth traits in goat. African Journal of Biotechnology, 11(82), 14738-14748.

Qiong, W., Chao, F., Wu-Jun, L., Yi, F., & Shi-Gang, Y. (2011). A novel mutation at exon 4 of IGF-1 gene in three indigenous goat breeds in China. Asian J. Anim. Vet. Adv, 6(6), 627-635.

Yeh, F. C., Yang, R. C., Boyle, T. B. J., Ye, Z. H., Mao, J. X., Yang, R. C., ... & Ye, Z. H. (1997). POPGENE, the user-friendly shareware for population genetic analysis.

Zhang, C., Zhang, W., Luo, H., Yue, W., Gao, M., & Jia, Z. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian-Australasian Journal of Animal Sciences, 21(8), 1073-1079.

Türkiye'de Yetiştirilen Bazı Keçi Irklarında İnsülin Benzeri Büyüme Faktörleri-1 (IGF-1) Geni Polimorfizm

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