Insulin-Like Growth Factor 1 (IGF-1) Gene Polymorphism in Some Goat Breeds Reared in Turkey
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https://doi.org/10.5281/zenodo.8238374Keywords:
IGF-1, HaeIII polymorphism, goats, PCR, RFLPAbstract
The purpose of this study is to determine HaeIII polymorphism in the exon 4 and intron 4 region of the Insulin-like Growth Factors -1 (IGF-1) gene which the regulation of tissue growth and muscle development in 6 different goat breeds reared in Turkey. HaeIII polymorphism in IGF-1 gene (363 bp) was investigated by Restriction Fragment Length Polymorphism PCR-RFLP methodology in a total of 303 goats including 52 Hair, 51 Angora, 50 Kilis, 50 Honamlı, 50 Halep, and 50 Saanen breeds.
A and B allele frequencies in Hair, Angora, Honamlı, Halep, Saanen and Kilis breeds were found to be 0.3558 and 0.6442, 0.5784 and 0.4216, 0.2100 and 0.7900, 0.7300 and 0.2700, 0.7800 and 0.2200, 0.2900 and 0.7100, respectively. AA, AB and BB genotype frequencies were found to be 0.250, 0.212 and 0.538 (P<0.05), 0.451, 0.255 and 0.294 (P<0.05), 0.060, 0.300 and 0.640 (P>0.05), 0.600, 0.260 and 0.140 (P<0.05), 0.700, 0.160 and 0.140 (P<0.05), 0.200, 0.180 and 0.620 (P<0.05) respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4901 and 0.5099, and AA, AB and BB genotype frequencies were found to be 0.376, 0.228 and 0.396 (P<0.05). It was determined that populations except for Honamlı goats were in not Hardy-Weinberg equilibrium when both the breed and the general evaluation were made in terms of HaeIII polymorphism in the exon 4 region of the IGF-1 gene (P<0.05).
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