Nigella Sativa Oil Improves Oxidative Stress, Inflammation and Changes in Neurotransmitter Structures in Fructose-Induced Metabolic Syndrome
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https://doi.org/10.5281/zenodo.8416168Keywords:
BDNF, Cytokines, Metabolic syndrome, Nigella sativa oil, Neurotransmitter, Oxidative stressAbstract
Metabolic syndrome (MetS) causes inflammation in adipose tissue along with oxidative stress and cellular damage. We aimed to investigate the protective effects and biochemical mechanisms of nigella sativa oil on MetS caused by excessive fructose intake in rats. 21 male Sprague-Dawley rats were used in the study. Rats; were divided into groups as control group, metabolic syndrome group and nigella sativa oil group. While the rats in the control group were fed only tap water and pellet food, the rats in the experimental group were fed with tap water with 10% fructose and pelleted diet for 10 weeks ad libitum. Nigella sativa group was given 0.1 ml of nigella sativa oil daily by oral tube for 4 weeks. After the study was completed, the rats were decapitated and serum biochemistry parameters, BDNF, neurotransmitters, cytokine and antioxidant levels were measured. Serum glucose, insulin, insulin resistance (HOMA-IR) and lipid profile levels increased with metabolic syndrome were significantly reduced with nigella sativa oil. Nigella sativa oil (NSO) regulated brain-derived neurotrophic factor (BDNF), 5-hydroxyindole acetic acid (5-HIAA), 5-hydroxytryptamine (5-HT), nor-adrenaline (NA), adrenaline (AD), dopamine (DA), tumor necrosis factor-α (Tnf-α), interleukin-6 (IL-6), total antioxidant status (TAS) and total oxidant status (TOS) parameters in the fructose-induced metabolic syndrome rat model. NSO was found to be a promising application option in preventing the development of oxidative stress and inflammatory damage caused by metabolic syndrome and in elucidating the complex mechanisms of neurotransmitters.
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