Timerosal ve Metabolitlerinin Uyarılmış İnsan Lenfositlerinin (-S9/+S9) Hücre Çoğalma Kinetiği ve Mikronükleus Frekansı Üzerindeki İn Vitro Etkileri


Özet Görüntüleme: 63 / PDF İndirme: 42

Yazarlar

DOI:

https://doi.org/10.5281/zenodo.8189561

Anahtar Kelimeler:

Thimerosal, Micronucleus, human lymphocytes, cytokinesis block proliferation index

Özet

Thimerosal, aşılarda ve biyomedikal preparatlarda etilcıva içeren bir koruyucudur. Timerosalin insan periferik kan lenfositlerinde in vitro genotoksisite reaksiyonları hakkında çok az şey bilinmektedir. Çalışmada, kültüre edilmiş insan periferik kan lenfositlerinde S9 fraksiyonu olan ve olmayan koşullarda kısa süreli timerosal toksisitesi araştırılmıştır. Sitokinez-Blok Mikronükleus Testi, genotoksisitenin değerlendirilmesi için yararlı bir tekniktir. Kültüre edilmiş insan periferik kan lenfosit hücreleri, S9 fraksiyonu olmayan koşullar altında 37oC'de 72 saat boyunca 0.2ug/ml-0.6ug/ml konsantrasyonlarında timerosal ile inkübe edilmiştir. Kültüre edilmiş insan periferik kan lenfosit hücreleri, S9 fraksiyonu içeren koşullar altında 37°C'de 3 saat boyunca 0.2ug/ml-0.6ug/ml konsantrasyonlarında timerosal ile inkübe edilmiştir. Timerosal, hem S9 fraksiyonu olan hem de olmayan koşullar altında sitokinez ile bloke edilmiş lenfositlerde doza bağlı bir şekilde mikronükleus (MN) oluşumunu indüklemiştir. Ayrıca timerosalin 0,2 μg/mL dozu dışında, negatif kontrol ile karşılaştırıldığında tüm dozlarda sitokinez blok proliferasyon indeksinde önemli ölçüde düşüş gözlenmiştir.

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Yayınlanmış

25.07.2023

Nasıl Atıf Yapılır

Eke, D., & Çelik, A. (2023). Timerosal ve Metabolitlerinin Uyarılmış İnsan Lenfositlerinin (-S9/+S9) Hücre Çoğalma Kinetiği ve Mikronükleus Frekansı Üzerindeki İn Vitro Etkileri. Euroasia Journal of Mathematics, Engineering, Natural & Medical Sciences, 10(28), 13–22. https://doi.org/10.5281/zenodo.8189561

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