In vitro Effects of Thimerosal and Its Metabolites on Cell Proliferation Kinetics and micronuclei frequency of Stimulated Human  Lymphocytes (-S9/+S9)

Dilek Eke; Ayla Çelik

doi:10.5281/zenodo.8189561

Authors

Dilek Eke Department of Public Health, The Ministry of Health, Malatya, Turkey https://orcid.org/0000-0001-7284-0410
Ayla Çelik Mersin University, Faculty of Science and Letters, Department of Biology, Mersin/Turkey https://orcid.org/0000-0002-0127-3639

DOI:

https://doi.org/10.5281/zenodo.8189561

Keywords:

Thimerosal, Micronucleus, human lymphocytes, cytokinesis block proliferation index

Abstract

Thimerosal is an ethylmercury-containing preservative in vaccines and biomedical preparations. Little is known about the genotoxicity reactions of thimerosal in human peripheral blood lymphocytes in vitro. In the study, short-term thimerosal toxicity was investigated in cultured human peripheral blood lymphocytes under conditions with and without S9 fraction. Cytokinesis-Block Micronucleus Test is a useful technique for the assessment of genotoxicity. Cultured human peripheral blood lymphocyte cells were incubated with 0.2µg/ml-0.6µg/ml concentrations of thimerosal for 72 h at 37^oC under conditions without S9 fraction. Cultured human peripheral blood lymphocyte cells were incubated with 0.2µg/ml-0.6µg/ml concentrations of thimerosal for 3 h at 37^oC under conditions with S9 fraction. Thimerosal induced the formation of micronuclei (MN) in a dose-dependent manner in the cytokinesis-blocked lymphocytes under conditions both with and without S9 fraction. Besides, thimerosal significantly decreased the cytokinesis block proliferation index in all the doses when compared with negative control, except at the dose of 0.2 μg/mL compared with negative control.

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In vitro Effects of Thimerosal and Its Metabolites on Cell Proliferation Kinetics and micronuclei frequency of Stimulated Human Lymphocytes (-S9/+S9)

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