EVALUATION OF ANTIMICROBIAL EFFECT OF PHOTO-ACTIVATED DISINFECTION ON Enterococcus Faecalis and Candida Albicans

Abstract

Objective In order to provide disinfection in the root canal system, it is necessary to clean root walls and root canal with antimicrobial agents in addition to effective instrumentation. Aim of this in vitro study was compare antimicrobial effects of photo-activated disinfection (PAD) and calcium hydroxide (CH) on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans). Material and Methods Microbial strains used in the study were obtained from Ankara Refik Saydam Hıfzıssıhha Institute Culture Collection. In study, C. Albicans and E. faecalis microorganisms were used. PAD and CH were used as antimicrobial agent. Six petri dishes were prepared for each group (negative control, PAD and CH groups). A cavity was formed on the agar in each petri dish to be 5 mm in diameter and 2 mm in depth. PAD and CH were applied to agar plates of each microorganism at sterile conditions. Prepared 18 petri dishes were incubated at 37 °C for 24 hours. Then, microbial inhibition diameters of the groups were measured. Statistical analysis of data was evaluated with t-test. Significance level (p) was considered to be 0.05 in the application of the test. Results For E. faecalis and C. albicans the highest inhibition zone diameters were measured in PAD group. PAD showed higher antimicrobial effect than CH against E. faecalis and C. albicans (p<0.001). Antimicrobial effect of CH and PAD against C. albicans was found statistically higher than E. faecalis (p<0.001). Conclusion Within the limitations of this in vitro study, it was found that antimicrobial effect of PAD against C. albicans and E. faecalis is higher than CH.