A RESEARCH ON MICROPROPAGATION OF PIXY ROOTSTOCK
(A RESEARCH ON MICROPROPAGATION OF PIXY ROOTSTOCK
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Yazar
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Mine PAKYÜREK, Serra HEPAKSOY
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Türü |
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Baskı Yılı |
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2020
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Sayı |
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8
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Sayfa |
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146-159
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Özet
Clonal rootstocks have ability to produce fruits in a very short period after planting, therefore
are preferred by farmers in establishing an orchard. Genetically identical origin and diseasefree
rootstocks can be obtained by in vitro techniques under laboratory conditions. The purpose
of study was to investigate the reproduction possibilities of Pixy, an important plum clone
rootstock used in plum and apricot cultivation, using the tissue culture techniques. The shoot
tips of Pixy rootstocks were used as explants and Murashige-Skoog (MS) containing 30 g L-1
sucrose and 7 g L-1 agar was used as the nutrient medium. The shoot tips cleaned and placed on
MS medium were transferred into the culture room with well controlled temperature and
lighting. The samples were sub-cultured onto the same medium once in a month during shoot
formation and rooting stages. The number of explants, leaves and sprouting were recorded once
in a 20 days. The effects of seven different MS media were investigated during the shoot
multiplication stage. Shoot multiplication was at the highest rate when a medium enriched with
4.4 µM BAP + 0.49 µM IBA + 0.29 µM GA3 + 1mM PG. The efficiency of five different MS
media were investigated during the rooting stage. The results indicated that the highest rooting
performance of Pixy rootstocks can be obtained in the nutrient medium containing 1/2 MS + 2
mg/L NAA.
Anahtar Kelimeler
Pixy (Prunus institia L.), plum clone rootstock, in vitro propagation
Abstract
Clonal rootstocks have ability to produce fruits in a very short period after planting, therefore
are preferred by farmers in establishing an orchard. Genetically identical origin and diseasefree
rootstocks can be obtained by in vitro techniques under laboratory conditions. The purpose
of study was to investigate the reproduction possibilities of Pixy, an important plum clone
rootstock used in plum and apricot cultivation, using the tissue culture techniques. The shoot
tips of Pixy rootstocks were used as explants and Murashige-Skoog (MS) containing 30 g L-1
sucrose and 7 g L-1 agar was used as the nutrient medium. The shoot tips cleaned and placed on
MS medium were transferred into the culture room with well controlled temperature and
lighting. The samples were sub-cultured onto the same medium once in a month during shoot
formation and rooting stages. The number of explants, leaves and sprouting were recorded once
in a 20 days. The effects of seven different MS media were investigated during the shoot
multiplication stage. Shoot multiplication was at the highest rate when a medium enriched with
4.4 µM BAP + 0.49 µM IBA + 0.29 µM GA3 + 1mM PG. The efficiency of five different MS
media were investigated during the rooting stage. The results indicated that the highest rooting
performance of Pixy rootstocks can be obtained in the nutrient medium containing 1/2 MS + 2
mg/L NAA.
Keywords
Pixy (Prunus institia L.), plum clone rootstock, in vitro propagation
