Hızlı Erişim


Bu Dergi DOI ve Crosscheck üyesidir


Abstract


ASSESSMENT OF GENETIC DIVERSITY AMONG Vicia sativa L. CULTIVARS BY TOTAL PROTEIN PROFILE, CYTOLOGICAL ANALYSIS AND MOLECULAR CHARACTERIZATION

Faba bean (Vicia faba L.) is an important food and feed legume because of the nutritional value of its seed protein and starch content, high biomass, and resistant environmetal conditions. It is commonly grown as a crop for human consumption. In this study, seeds of 5 cultivars of Vicia faba were evaluated for the nuclear DNA, chromosome count, and total protein amount. 5 different vicia faba seeds were cultured on MS basal medium at a temperature of 23°C ± 2°C under a 16 h light photoperiod with the light intensity of 97 µmol m-2 s-1 for 2 weeks. The amount of nuclear DNA was measured by flow cytometry analysis with leaf samples. Chromosome count was made by taking samples from root tips of 5 different faba bean (Vicia faba) cultivars. SDS PAGE analysis was produced bands between 140 kDa and 25 kDa. SDS PAGE also analysis displayed the accumulation of proteins in leaves. In terms of SDS PAGE analysis, Kansur exhibited the most abundant protein, followed by Tarım Beyazı and Ankara Moru. Total protein amount is the lowest in Ankara Pembesi. The amount of nuclear DNA content ranged from 3.53 pg to 11.11 pg. According to flow cytometry results, there was no significant variation between nuclear DNA contents of Kansur and Tarım Beyazı, Ankara Pembesi and Ankara Moru. The four Vicia faba plants showed the same chromosome numbers as 2n= 2x= 12. However, 2n= 2x= 14 number was detected in the root tips of the Kansur. Kansur displayed that nucleat DNA content and protein accumulation was higher the other cultivars.



Keywords
Faba bean (Vicia subsp.), Flow cytometry, Chromosome count, SDS PAGE



Kaynakça Maxted, N. (1993), ‘A phenetic investigation of Vicia L. subgenus Vicia (Leguminosae, Vicieae),’ Botanical Journal of the Linnean Society, 111, 155-182. Ye, Y., Lang, L., Xia, M., Tu, J., (2003),’ Faba beans in China,’ China Agriculture Press, Beijing, 1:15. Terzopoulos PJ, Bebeli PJ (2008), Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers,’ Field Crops Res, 108:39–44. Johnston JS, Bennett MD, Rayburn AL, Galbraith DW, Price HJ (1999),’ Reference standards for determination of DNA content of plant nuclei,’ Am J Bot, 86:609–613. Signor CL, Gallardo K, Prosperi JM, Salon C, Quillien L, Thompson R and Duc G. (2005), ‘Genetic diversity for seed protein composition in Medicago truncatula,’ Plant Genetic Resources, 3(1):59–71. Sammour R, Mustafa A, Badr S and Taher W. (2007),’ Genetic variations in accessions of Lathyrus sativus L.,’ Acta Bot. Croat. 66 (1): 1–13. Ochatt S, Sangwan R, (2008), ‘In vitro shortening of generation time in Arabidopsis thaliana,’ Plant Cell Tissue Organ Cult, 93:133–137. Agayev YM (1998), ‘ Advanced squash methods for investigation of plant chromosomes,’ Journal of Heredity, 79(4):225-238. Laemmli UK (1970), ‘Cleavage of structural proteins during the assembly of the head of bacteriophage T4,2 Nature, 227:680–685. Cooper, J. W., Wilson, M. H., Derks, M. F. L., Smit, S., Kunert, K. J., Cullis, C., & Foyer, C. H. (2017), ‘Enhancing faba bean (Vicia faba L.) genome resources,’ Journal of Experimental Botany, 68(8), 1941-1953. https://doi.org/10.1093/jxb/erx117 Kubaláková, M., Valárik, M., Bartoš, J., Vrána, J., Cíhalíková, J., Molnár-Lang, M., Dolezel, J.: (2003), ‘Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry,’ Genome 46: 893-905.

Gelişmiş Arama


Duyurular

    ***********************

    DEĞERLİ BİLİM 

    İNSANLARI!

    mail mail mail mail mail

    Dergimizin Mayıs sayısı 

    (27.05.2021)

    yayınlanmıştır.

    mail mail mail mail mail

    DEĞERLİ BİLİM 

    İNSANLARI!

    mail mail mail mail mail

    Dergimizin

    Temmuz Sayısı 

    İçin Makalenizi  

    Sisteme Yükleyebilirsiniz.

    mail mail mail mail mail

     



Adres :Göztepe Mah., Beykoz, İstanbul/TURKEY
Telefon :+90 555 005 92 85 Faks :+90 216 606 32 75
Eposta :info@euroasiajournal.org

Web Yazılım & Programlama Han Yazılım Bilişim Hizmetleri